Anti-CD47 Antibody [B6H12.2] (A250728)

Anti-CD47 Antibody [B6H12.2]

Mouse monoclonal [B6H12.2] antibody to CD47.

This antibody reacts with Ig domain of CD47 protein. It has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.

Functional Studies, WB, Flow Cytometry, IF

WB: 1-2 µg/ml, Flow Cytometry: 1-2 µg/million cells, IF: 1-2 µg/ml

Human

Intact CD47 purified from placenta.

Mouse

Monoclonal

B6H12.2

IgG1

kappa

Protein A/G chromatography.

200 µg/ml

~50 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD47 Antibody [B6H12.2] - BSA and Azide free (A253908).

Antigenic surface determinant protein OA3, IAP, Integrin-associated protein, Leukocyte surface antigen CD47, MER6, Protein MER6