Western Blot - Anti-CD47 Antibody [ARC0584] (A81040)
Western blot analysis of extracts of various cell lines, using Anti-CD47 Antibody [ARC0584] (A81040) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Enhanced Kit (RM00021). Exposure time: 90s.
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