Mouse monoclonal [UCHL1] antibody to CD45RO (FITC).
Specificity
This antibody recognises the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood
Reactivity
Human, Chimpanzee, Marmoset
Immunogen
Human IL-2 dependent T cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
UCHL1
Isotype
IgG2a
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
100 µg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.
General Notes
Mouse anti Human CD45RO monoclonal antibody, clone UCHL1 recognizes the low molecular weight isoform (180 kDa) of the leucocyte common antigen (LCA). The antigen is expressed by a functional subset of T cells with memory phenotype.In peripheral blood Mouse anti Human CD45RO, clone UCHL1 stains 40-80% of lymphocytes and all monocytes and granulocytes.