This antibody recognises the human leukocyte common antigen CD45, expressed on all cells of haematopoietic origin except for erythrocytes. The isolation and characterisation of this cell line was carried out with the help of funds supplied by the Medical Research Council, United Kingdom.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Human
Immunogen
Human T lymphocytes.
Host
Rat
Clonality
Monoclonal
Clone ID
YTH24.5
Isotype
IgG2b
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light.
General Notes
Rat anti Human CD45 antibody, clone YTH24.5 recognizes the human leukocyte common antigen CD45, expressed on all cells of haematopoietic origin except for erythrocytes. The isolation and characterisation of this cell line was carried out with the help of funds supplied by the Medical Research Council, United Kingdom.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200