This antibody recognises the human leukocyte common antigen CD45, expressed on all cells of haematopoietic origin except for erythrocytes. The isolation and characterisation of this cell line was carried out with the help of funds supplied by the Medical Research Council, United Kingdom.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human
Immunogen
Human T lymphocytes.
Host
Rat
Clonality
Monoclonal
Clone ID
YTH24.5
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Rat anti Human CD45 antibody, clone YTH24.5 recognizes the human leukocyte common antigen CD45, expressed on all cells of haematopoietic origin except for erythrocytes. The isolation and characterisation of this cell line was carried out with the help of funds supplied by the Medical Research Council, United Kingdom.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
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