Anti-CD45 Antibody [MAC323] (A281019)

Anti-CD45 Antibody [MAC323]

Mouse monoclonal [MAC323] antibody to CD45.

This antibody recognises an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). recognises a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323-ve animals rather than loss of the CD45 antigen. Cross-breeding positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern.

IHC-P, IHC-Fr, Flow Cytometry, IP, IHC-R

Flow Cytometry: 1:25 - 1:100, IHC-Fr: 1:10, IHC-P: 1:10, IHC-R 1: 1:10

Porcine

Porcine Peripheral Blood Lymphocytes.

Mouse

Monoclonal

MAC323

IgG2a

Protein A affinity chromatography of tissue culture supernatant.

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Pig CD45 allotypic variant antibody, clone MAC323 recognizes an allotypic determinant of porcine CD45, also known as the leucocyte common antigen (LCA). Mouse anti Pig CD45 allotypic variant antibody, clone MAC323 has been shown to recognize CD45 in pigs of the Large White and German Landrace strains. Another antibody, clone K252.1E4, recognizes a monomorphic determinant on porcine CD45. When K252.1E4 is used in conjunction with MAC323, it can be shown that MAC323 stains a subset of the leukocytes that are detected by K252.1E4; indicating a loss of the epitope in MAC323-ve animals rather than loss of the CD45 antigen. Cross-breeding positive vs. negative animals indicate that the MAC323 gene is inherited in a simple Mendelian autosomal dominant pattern (Binns et al. 1995).

L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200