This antibody recognises an epitope common to all porcine CD45 isoforms. Clone K252.1E4 immunoprecipitates three polypeptides of 226, 210 and 190 kDa from preparations of porcine peripheral blood mononuclear cells and shows a broad reactivity pattern with both lymphoid and myeloid cells.
Applications
Flow Cytometry, IHC-Fr, IF
Dilutions
Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Porcine
Immunogen
Porcine Peripheral Blood Lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
K252.1E4
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
CD45 is also known as leukocyte common antigen (LCA).
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
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