Anti-CD45 Antibody [IBL-3/16] (A280683)

Anti-CD45 Antibody [IBL-3/16]

Rat monoclonal [IBL-3/16] antibody to CD45.

This antibody recognises mouse Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kDa single pass type I transmembrane glycoprotein belonging to the protein -tyrosing phosphatase family. CD45 has two fibronectin type-III domains and two tyrosine protein phosphatase domains (UniProt:: P06800).Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region. Rat anti Mouse CD45 antibody, clone IBL-3/16 is expected to recognize all isoforms of murine CD45.Rat anti Mouse CD45 antibody, clone IBL-3/16 has been used successfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques (Kondo et al. 2011, Cuadros et al. 2006).

IP, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 - 1:100, IHC-Fr 1: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse

This antibody does not cross react with Rat, Human, or Chicken

Purified B cells from mouse lymph nodes.

Rat

Monoclonal

IBL-3/16

Protein G affinity chromatography of tissue culture supernatant.

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD45 antibody, clone IBL-3/16 recognizes murine Receptor-type tyrosine-protein phosphatase C, also known as CD45, Leukocyte common antigen, T200 or Lymphocyte antigen 5 (Ly5). CD45 is a 1291 amino acid ~175kDa single pass type I transmembrane glycoprotein belonging to the protein -tyrosing phosphatase family. CD45 has two fibronectin type-III domains and two tyrosine protein phosphatase domains (UniProt:: P06800).Multiple isoforms are generated by alternative splicing with isoforms having differing deletions in the N-terminal region (Saga et al. 1987). Rat anti Mouse CD45 antibody, clone IBL-3/16 is expected to recognize all isoforms of murine CD45.Rat anti Mouse CD45 antibody, clone IBL-3/16 has been used successfully for the identification of CD45 in murine samples using Immunohistochemical (both cryo and FFPE), immunofluorescence and western blotting techniques (Kondo et al. 2011, Cuadros et al. 2006).

L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200