This antibody recognizes an extracellular epitope on all isoforms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high levels on all cells of hematopoietic origin, except erythrocytes and platelets.
Applications
Flow Cytometry, IP, WB, IHC-P, IHC-Fr, ICC
Dilutions
WB: 1 µg/ml; Non-reducing conditions, band around 200-250 kDa; Positive Control: JURKAT human leukemia T-cell lysate, Kg-1a human leukemia cell lysate; Non-reducing conditions, 6% separating gel, Flow Cytometry: 1-4 µg/ml.
Reactivity
Human, Non-Human Primates
Immunogen
Unfortunately, this information is considered to be commercially sensitive.
Host
Mouse
Clonality
Monoclonal
Clone ID
HI30
Isotype
IgG1
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein G chromatography.
Concentration
1 mg/ml
Predicted MW
180-220 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
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