Anti-CD45 Antibody [F10-89-4] (PE-Cyanine 5.5) (A280407)

$515

Mouse monoclonal [F10-89-4] antibody to CD45 (PE-Cyanine 5.5) for Flow Cytometry.

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-CD45 Antibody [F10-89-4] (PE-Cyanine 5.5)
Description
Mouse monoclonal [F10-89-4] antibody to CD45 (PE-Cyanine 5.5).
Specificity
This antibody recognises the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: 1:10 - 1:50, IHC-Fr: 1:500 - 1:1,000, IHC-P: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human
Immunogen
Human T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
F10-89-4
Isotype
IgG2a
Conjugate

PE-Cyanine 5.5

Excitation: 496nm, Emission: 667nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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