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Anti-CD45 Antibody [F10-89-4] - BSA and Azide free (A280410)

$830

Mouse monoclonal [F10-89-4] antibody to CD45 for Flow Cytometry, IHC-Fr, IP and IHC-P.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-CD45 Antibody [F10-89-4] - BSA and Azide free
Description
Mouse monoclonal [F10-89-4] antibody to CD45.
Specificity
This antibody recognises the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes.
Applications
Flow Cytometry, IHC-Fr, IP, IHC-P
Dilutions
Flow Cytometry: 1:10 - 1:50, IHC-Fr: 1:500 - 1:1,000, IHC-P: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Human
Immunogen
Human T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
F10-89-4
Isotype
IgG2a
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline.
Storage
Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD45 antibody, clone F10-89-4 recognizes the human CD45 cell surface antigen, also known as leucocyte common antigen (LCA). CD45 is a complex molecule existing in a number of isoforms. Antibodies recognizing a common epitope on all of these isoforms are termed CD45 whilst those recognizing only individual isoforms are termed CD45RA or CD45RO etc. Mouse anti Human CD45 antibody, clone F10-89-4 reacts with all forms of CD45 expressed by all haematopoietic cells, except erythrocytes, having a higher level of expression on lymphocytes than on granulocytes. Mouse anti Human CD45 antibody, clone F10-89-4 is routinely tested in flow cytometry on human peripheral blood leucocytes.
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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