Western Blot - Anti-CD45 Antibody [ARC0459] (A308608)
Western blot analysis of extracts of various cell lines, using Anti-CD45 Antibody [ARC0459] (A308608) at 1:200 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 180s.
Immunohistochemistry analysis of paraffin-embedded human colon tissue using Anti-CD45 Antibody [ARC0459] (A308608) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver using Anti-CD45 Antibody [ARC0459] (A308608) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using Anti-CD45 Antibody [ARC0459] (A308608) at a dilution of 1:20(40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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