Anti-CD45 Antibody (A306074)

Western blot analysis of Jurkat, using Anti-CD45 Antibody (A306074) at 1:1,500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.

Immunofluorescence analysis of Jurkat cells using Anti-CD45 Antibody (A306074) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of mouse spleen cells using Anti-CD45 Antibody (A306074) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Immunofluorescence analysis of rat spleen cells using Anti-CD45 Antibody (A306074) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.