Mouse monoclonal [2D1] antibody to CD45 (PE-Cyanine 5).
Specificity
This antibody reacts with an extracellular epitope of all alternative forms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high levels on all cells of hematopoietic origin, except from erythrocytes and platelets.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human peripheral blood mononuclear cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
2D1
Isotype
IgG1
Light Chains
kappa
Conjugate
PE-Cyanine 5
Excitation: 496nm, Emission: 667nm
Purification
This antibody is conjugated with tandem dye PE-Cyanine 5 under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
180-220 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD45 Antibody [2D1] (PE-Cyanine 5) (4µl reagent per 100µl of peripheral whole blood).
Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD45 Antibody [2D1] (PE-Cyanine 5) (4µl reagent per 100µl of peripheral whole blood).
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