This antibody reacts with an extracellular epitope of all alternative forms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high levels on all cells of hematopoietic origin, except from erythrocytes and platelets.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Human peripheral blood mononuclear cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
2D1
Isotype
IgG1
Light Chains
kappa
Conjugate
APC
Excitation: 645nm, Emission: 660nm
Purification
This antibody is conjugated with Allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
180-220 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Synonyms
L-CA, Leukocyte common antigen, PTPRC, Receptor-type tyrosine-protein phosphatase C, T200
Flow cytometric analysis of human peripheral whole blood stained using Anti-CD45 Antibody [2D1] (APC) (10µl reagent per 100µl of peripheral whole blood).
Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis of human peripheral whole blood using Anti-CD45 Antibody [2D1] (APC) (10µl reagent per 100µl of peripheral whole blood).
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