This antibody recognises the rabbit CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by all leucocytes. In immunohistochemical staining the antibody labels the medullary area strongly and the cortical area weakly. Mouse anti Rabbit CD44 antibody, clone W4/86 has been reported as being suitable for use in Western blotting under non-reducing conditions.
Applications
IP, Flow Cytometry, IHC-Fr
Dilutions
Flow Cytometry: 1:25 - 1:200, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rabbit
Immunogen
RL-5 T cell line glycoproteins.
Host
Mouse
Clonality
Monoclonal
Clone ID
W4/86
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rabbit CD44 antibody, clone W4/86 recognizes the rabbit CD44 cell surface antigen, a ~95 kDa glycoprotein expressed by all leucocytes. In immunohistochemical staining the antibody labels the medullary area strongly and the cortical area weakly.Mouse anti Rabbit CD44 antibody, clone W4/86 has been reported as being suitable for use in Western blotting under non-reducing conditions.