This antibody reacts with an extracellular antigen of both cell surface-expressed and soluble form of CD44 antigen, a 80-95 kDa transmembrane glycoprotein of the hyaladherin family, present on the surface of most cells and tissues, including: leukocytes, endothelial cells, mesenchymal cells, etc. It is negative on platelets and hepatocytes.
Applications
Flow Cytometry, IP, WB, ELISA
Dilutions
Flow Cytometry: 2-6 µg/ml, WB: 2 µg/ml, 60 min on vertical incubator; Positive Control: Kg-1a human leukemia cell lysate, Non-reducing conditions.
Reactivity
Human
Immunogen
Leukocytes from a patient suffering from a LGL Type leukemia.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-85
Isotype
IgG2b
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
80-95 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.