Western blot analysis of extracts of various cell lines, using Anti-CD44 Antibody (A93018) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Western blot analysis of extracts from normal (control) and CD44 knockout (KO) HeLa cells, using Anti-CD44 Antibody (A93018) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded human esophageal cancer using Anti-CD44 Antibody (A93018) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using Anti-CD44 Antibody (A93018) at a dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
Confocal immunofluorescence analysis of A431 cells using Anti-CD44 Antibody (A93018) at a dilution of 1:200. DAPI was used to stain the cell nuclei (blue).
Immunofluorescence analysis of RAW264. 7 cells using Anti-CD44 Antibody (A93018) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
Confocal immunofluorescence analysis of HeLa cells using Anti-CD44 Antibody (A93018) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).
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