This antibody recognises the bovine Tumor necrosis factor receptor superfamily member 5, also known as CD40 or B-cell surface antigen CD40. CD40 is a 260 amino acid single pass type 1 transmembrane glycoprotein with a predicted molecular mass of 31 kDa. Bovine CD40 also has an N terminal signal peptide of 20 amino acids. Bovine CD40 is expressed by B lymphocytes and a subset of T lymphocytes.CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 1x106 cells in 100µl
Reactivity
Bovine, Sheep
Host
Mouse
Clonality
Monoclonal
Clone ID
IL-A156
Isotype
IgG1
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Gently mix after reconstitution.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Bovine CD40 antibody, clone IL-A156 recognizes the bovine Tumor necrosis factor receptor superfamily member 5, also known as CD40 or B-cell surface antigen CD40. CD40 is a 260 amino acid single pass type 1 transmembrane glycoprotein with a predicted molecular mass of 31 kDa. Bovine CD40 also has an N terminal signal peptide of 20 amino acids. Bovine CD40 is expressed by B lymphocytes and a subset of T lymphocytes.CD40 plays an important role in the differentiation of B lymphocytes into effector cells, and is also involved in interactions between T and B lymphocytes.