Recombinant Anti-CD40 Antibody [DM100] - BSA and Azide free (A318605)

ELISA plates were pre-coated with Recombinant Human CD40 Protein (Fc Chimera 6xHis Tag) (A318393) at 2 µg/ml (100 µl/well) which can bind Anti-CD40 Antibody [DM100] - BSA and Azide free (A318605) in a linear range of 0.64-80 ng/ml.

A. Flow cytometry analysis with Anti-CD40 Antibody [DM100] - BSA and Azide free (A318605) on Expi293 cells transfected with human CD40 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).

Flow cytometry analysis of antigen binding of Anti-CD40 Antibody [DM100] - BSA and Azide free (A318605). (A) Anti-CD40 Antibody [DM100] - BSA and Azide free (A318605) does not bind to Jurkat cells that do not express CD40. (B) A clear peak shift of Anti-CD40 Antibody [DM100] - BSA and Azide free (A318605) was seen compared to the control when incubated with CD40-expressing Raji cells, indicating strong binding of Anti-CD40 Antibody [DM100] - BSA and Azide free (A318605) to CD40. Antibodies were incubated at 2 µg/ml.

Western Blot analysis of Raji (human Burkitt’s lymphoma B lymphocyte), whole cell lysate using Anti-CD40 Antibody [DM100] - BSA and Azide free (A318605) at 1/100 dilution. Secondary: Goat Anti-Rabbit IgG H&L (HRP) at 1/5000 dilution. Predicted band size: 30 kDa. Observed band size: 45 kDa.