Anti-CD40 Antibody [3/23] (PE) (A278860)

Anti-CD40 Antibody [3/23] (PE)

Rat monoclonal [3/23] antibody to CD40 (PE).

This antibody recognises the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity of Rat anti Mouse CD40 antibody, clone 3/23 was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40.Rat anti Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.

Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl. The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

Mouse

Extracellular Domain of Mouse CD40 and the Fc portion of human IgG1.

Rat

Monoclonal

3/23

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD40 antibody, clone 3/23 recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity of Rat anti Mouse CD40 antibody, clone 3/23 was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40.Rat anti Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.

This product is for research use only. It is not intended for diagnostic or therapeutic use.