This antibody recognises the Mouse CD4 antigen, non polymorphic epitope. recognises with CD4 transfectants. Rat anti Mouse CD4 antibody, clone YTS177.9 also blocks MHC-II dependant T-cell responses in vitro and in vivo and induces tolerance and Cobbold et al. 1990).
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells or 100µl of whole blood.The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/BUF041B).
Reactivity
Mouse
Immunogen
Mouse spleen cells.
Host
Rat
Clonality
Monoclonal
Clone ID
YTS177.9
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with the Mouse CD4 antigen, non polymorphic epitope (Qin et al. 1990). Rat anti Mouse CD4 antibody, Rat anti Mouse CD4 antibody, clone YTS177.9 reacts with CD4 transfectants (Cobbold et al. 1990).Rat anti Mouse CD4 antibody, clone YTS177.9 also blocks MHC-II dependant T-cell responses in vitro and in vivo and induces tolerance (Qin et al. 1990) and Cobbold et al. 1990).