Anti-CD4 Antibody [YKIX302.9] (A282044)

Anti-CD4 Antibody [YKIX302.9]

Rat monoclonal [YKIX302.9] antibody to CD4.

This antibody recognises the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992] along with clone CA13.1E4. Rat anti Dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administered in vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model. Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets.

Flow Cytometry, IHC-Fr

Flow Cytometry: 1:50 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Canine

Canine concanavilin A activated T cell blasts.

Rat

Monoclonal

YKIX302.9

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Dog CD4 antibody, clone YKIX302.9, is a monoclonal antibody specific for the canine CD4 cell surface antigen. Clone YKIX302.9 was clustered at the first Canine Leukocyte Antigen Workshop (CLAW) [Cobbold et al. 1992] along with clone CA13.1E4. Rat anti Dog CD4 antibody, clone YKIX302.9 partially depletes circulating T lymphocytes when administered in vivo, but alone is not sufficient to prolong allograft survival in a canine transplant model (Watson et al. 1993). Uniquely amongst mammals, canine CD4 is expressed by neutrophils as well as by lymphocyte subsets (Moore et al. 1992).

B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, TNFRSF5, Tumor necrosis factor receptor superfamily member 5