Anti-CD4 Antibody [W3/25] - BSA and Azide free (A281959)

Anti-CD4 Antibody [W3/25] - BSA and Azide free

Mouse monoclonal [W3/25] antibody to CD4.

This antibody recognises the rat CD4 cell surface glycoprotein, a ~55 kDa molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction. Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes.

IHC-Fr, IHC-P, Flow Cytometry, IF, Functional Assays

Flow Cytometry: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Rat Thymocyte Membrane Glycoproteins.

Mouse

Monoclonal

W3/25

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Rat CD4 antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein, a ~55 kDa molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction.Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (Whiteland et al. 1995).Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes.

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4