Anti-CD4 Antibody [W3/25] (APC) (A281953)

Anti-CD4 Antibody [W3/25] (APC)

Mouse monoclonal [W3/25] antibody to CD4 (APC).

This antibody recognises the rat CD4 cell surface glycoprotein, a ~55 kDa molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction. Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes.

Flow Cytometry: 1:5 - 1:10, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Rat Thymocyte Membrane Glycoproteins.

Mouse

Monoclonal

W3/25

IgG1

Protein G affinity chromatography of tissue culture supernatant.

Reconstitution dependent.

Lyophilized

Reconstitute with 1 ml of distilled water.

Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.

Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Rat CD4 antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein, a ~55 kDa molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction.Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (Whiteland et al. 1995).Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes.

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

This product is for research use only. It is not intended for diagnostic or therapeutic use.