Anti-CD4 Antibody [W3/25] (A281956)

Anti-CD4 Antibody [W3/25]

Mouse monoclonal [W3/25] antibody to CD4.

This antibody recognises the rat CD4 cell surface glycoprotein, a ~55 kDa molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction. Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes.

IHC-P, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:100 - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Rat

Rat Thymocyte Membrane Glycoproteins.

Mouse

Monoclonal

W3/25

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Rat CD4 antibody, clone W3/25 recognizes the rat CD4 cell surface glycoprotein, a ~55 kDa molecule expressed by helper T cells and weakly by monocytes. This antibody inhibits proliferation and IL-2 production in the MLR reaction.Mouse anti Rat CD4 antibody, clone W3/25 has been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde) (Whiteland et al. 1995).Mouse anti Rat CD4 antibody, clone W3/25 is routinely tested in flow cytometry on rat splenocytes.

B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, TNFRSF5, Tumor necrosis factor receptor superfamily member 5