This antibody recognises the rat CD4 cell surface antigen, also known as W3/25 antigen or T-cell surface antigen T4/Leu-3. CD4 is a 430 amino acid ~55 kDa single pass type I transmembrane glycoprotein expressed by T helper cells, monocytes and macrophages.Clone OX-38 competes for binding with Mouse anti Rat CD4 antibody, clone W3/25 (MCA55R). Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 has been utilized successfully for the in vitro depletion of T helper cells.
Applications
IHC-Fr, Flow Cytometry, IP
Dilutions
Flow Cytometry: 1:100 - 1:200
Reactivity
Rat
Immunogen
MLR generated rat T cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-38
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 recognizes the rat CD4 cell surface antigen, also known as W3/25 antigen or T-cell surface antigen T4/Leu-3. CD4 is a 430 amino acid ~55 kDa single pass type I transmembrane glycoprotein expressed by T helper cells, monocytes and macrophages.Clone OX-38 competes for binding with Mouse anti Rat CD4 antibody, clone W3/25 (MCA55R).Mouse anti Rat CD4 (Domain 1) antibody, clone OX-38 has been utilized successfully for the in vitro depletion of T helper cells (Pusic et al. 2015).