This antibody recognises the rat CD4 cell surface antigen, a ~55kDa glycoprotein expressed by helper T cells and weakly by monocytes.recognises a different epitope on the CD4 molecule to Mouse anti Rat CD4 antibody, clone W3/25.
Applications
IHC-Fr, IF, Flow Cytometry, IP
Dilutions
Flow Cytometry: 1:10 - 1:50, Use 10µl of the suggested working dilution to label 106 cells in 100µl.
Reactivity
Rat
Immunogen
MLR generated rat T helper lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
OX-35
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat CD4 (domain 2) antibody, clone OX-35 recognizes the rat CD4 cell surface antigen, a ~55kDa glycoprotein expressed by helper T cells and weakly by monocytes.Mouse anti Rat CD4 (Domain 2) antibody, clone OX-35 recognizes a different epitope on the CD4 molecule to Mouse anti Rat CD4 antibody, clone W3/25.