This antibody recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes ("helper" T-cells) and also on monocytes, tissue macrophages, and granulocytes.
Applications
Flow Cytometry, WB, CyTOF
Dilutions
Flow Cytometry: 1-5 µg/ml; Positive Control: peripheral blood, HPB T cell line. This antibody is excellent antibody for FC application, WB: 1-2 µg/ml; Positive Control: peripheral blood, HPB T cell line, Non-reducing conditions.
Reactivity
Human
Immunogen
2 N-terminal domains of human CD4 fused to human IgG1 Fc.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-241
Isotype
IgG1
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.