This antibody recognizes an extracellular epitope of CD4 antigen, a 55 kDa transmebrane glycoprotein expressed on a subset of T lymphocytes ("helper" T-cells) and also on monocytes, tissue macrophages, and granulocytes. This antibody does not block Leu3a and OKT4 binding. This antibody blocks HIV-1 infection in cell to cell system.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
Reactivity
Human
Immunogen
Normal human blood lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
EM4
Isotype
IgG2a
Conjugate
FITC
Excitation: 490nm, Emission: 525nm
Purification
This antibody is conjugated with Fluorescein Isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.
Predicted MW
55 kDa
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!
Flow cytometric analysis of human peripheral whole blood stained with Anti-CD4 Antibody [EM4] (FITC) (4µl reagent per 100µl of peripheral whole blood).
Separation of human CD4 positive lymphocytes (red-filled) from CD4 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained with Anti-CD4 Antibody [EM4] (FITC) (4µl reagent per 100µl of peripheral whole blood).
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