Anti-CD4 Antibody [CC30] (A280025)

Anti-CD4 Antibody [CC30]

Mouse monoclonal [CC30] antibody to CD4.

This antibody recognises a ~50 kDa transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected. Mouse anti Bovine CD4, clone CC30 has successfully been used for immunohistochemical localization of CD4 on paraffin embedded material using zinc salt fixation. Additionally, clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques.

IHC-P, IP, Flow Cytometry, IHC-Fr

Flow Cytometry: 1:25 - 1:200, IHC-Fr: 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl.

Bovine, Bison

Bovine thymocytes.

Mouse

Monoclonal

CC30

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Mouse anti Bovine CD4 antibody, clone CC30 recognizes a ~50 kDa transmembrane molecule considered to be the bovine homologue of human CD4. The phenotype, tissue distribution and function of T-cells expressing the bovine CD4 antigen are similar to those in other species. However, expression on macrophages has not yet been detected.Mouse anti Bovine CD4, clone CC30 has successfully been used for immunohistochemical localization of CD4 on paraffin embedded material using zinc salt fixation (Cantón et al. 2013). Additionally, clone CC30 has been reported as being suitable for use on formal dichromate (FD5) fixed paraffin embedded tissue with amplification and antigen retrieval techniques (Gutierrez et al. 1999).

T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4