Anti-CD4 Antibody (A90503)

Western blot analysis of extracts of U-937 cells, using Anti-CD4 Antibody (A90503) at 1:500 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of mouse thymus using Anti-CD4 Antibody (A90503) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of rat thymus cells using Anti-CD4 Antibody (A90503) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).

Immunofluorescence analysis of Jurkat cells using Anti-CD4 Antibody (A90503) at a dilution of 1:100. DAPI was used to stain the cell nuclei (blue).