Anti-CD4 Antibody (A82715)

Name: Anti-CD4 Antibody
Brand: Antibodies.com
SKU: A82715
Price: 495.0 USD
Availability: InStock

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$495

Goat polyclonal antibody to CD4 for ELISA, WB, IHC and Flow Cytometry.

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Shipping Information

Freight/Packing Charges: $40

Dispatched from St. Louis, MO.

Lead Time: 4-6 business days.

Tags:

Endosomal Marker

Specifications

Name

Anti-CD4 Antibody

Description

Goat polyclonal antibody to CD4.

Applications

ELISA, WB, IHC, Flow Cytometry

Dilutions

ELISA: 1:128,000, WB: 0.3-1µg/ml, IHC: 4µg/ml, Flow Cytometry: 10µg/ml

Reactivity

Human

Immunogen

Synthetic peptide corresponding to the internal region of human CD4.

Sequence

C-KNKEVSVKRVTQDPK

Host

Goat

Clonality

Polyclonal

Isotype

IgG

Conjugate

Unconjugated

Purification

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Concentration

500 µg/ml

Molecular Weight

30 kDa

Predicted MW

31.3kDa

Product Form

Liquid

Formulation

Supplied in Tris Buffered Saline, pH 7.3, with 0.5% BSA and 0.02% Sodium Azide.

Storage

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.

Synonyms

B-cell surface antigen CD40, Bp50, CD40, CD40L receptor, CDw40, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4, TNFRSF5, Tumor necrosis factor receptor superfamily member 5

Isotype Controls

Goat IgG (A121671)

Suitable Secondaries

See all Anti-Goat IgG Secondaries →

Disclaimer

This product is for research use only. It is not intended for diagnostic or therapeutic use.

Scientific Validation DataValidation Data (4)

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Western Blot - Anti-CD4 Antibody (A82715)

CD4 expression in Human Spleen (A) and Bone Marrow (B) lysate analyzed by western blot. Cells were lysed in RIPA buffer and 35µg protein was run per lane. Primary incubation was performed with Anti-CD4 Antibody (A82715) at 1µg/ml (A) or 0.5µg/ml (B) and detected by chemiluminescence.

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Flow Cytometry - Anti-CD4 Antibody (A82715)

CD4 expression in Jurkat cells (blue line) analyzed by flow cytometry. Cells were fixed in PFA and permeabilized with 0.5% Triton. Staining was performed with Anti-CD4 Antibody (A82715) at 10µg/ml for 1 hour and Alexa Fluor 488 secondary antibody at 1µg/ml. Negative Control: Goat IgG Isotype Control (black line) followed by Alexa Fluor 488 secondary antibody.

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Immunohistochemistry - Anti-CD4 Antibody (A82715)

CD4 expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and antigen retrieval was achieved by heating in citrate buffer, pH 6. Staining was performed with Anti-CD4 Antibody (A82715) at 4µg/ml and revealed with horseradish peroxidase (HRP).

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Immunohistochemistry - Anti-CD4 Antibody (A82715)

Negative control for CD4 expression in Human Tonsil analyzed by immunohistochemistry. Tissue was paraffin-embedded, and staining procedure was performed in the absence of primary antibody.

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