This antibody recognises the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells. Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:5, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Chicken, Turkey
Immunogen
Chicken embryonic thymocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
2-35
Isotype
IgG2b
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Chicken CD4, clone 2-35 recognizes the chicken homologue of human CD4, a ~64 kDa cell surface protein expressed by thymocytes and a subset of T cells (Luhtala et al. 1993). Mouse anti Chicken CD4, clone 2-35 has been demonstrated to recognize turkey CD4 (Li et al. 1998).