Anti-CD3G Antibody [ARC2105] (A306075)

Western blot analysis of extracts of various cell lines, using Anti-CD3G Antibody [ARC2105] (A306075) at 1:1,000 dilution. The secondary antibody was Goat Anti-Rabbit IgG H&L Antibody (HRP) at 1:10,000 dilution. Lysates/proteins were present at 25µg per lane. The blocking buffer used was 3% non-fat dry milk in TBST. Detection was with a ECL Basic Kit. Exposure time: 120s.

Immunofluorescence analysis of Jurkat cells using Anti-CD3G Antibody [ARC2105] (A306075) at a dilution of 1:100 (40x lens). DAPI was used to stain the cell nuclei (blue).

Flow cytometry analysis of Jurkat cells, stained with Rabbit IgG isotype control (2.5 µg/ml, blue line) or Anti-CD3G Antibody [ARC2105] (A306075), (2.5 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Jurkat cells was used as blank control (red line).

Flow cytometry analysis of Raji cells, stained with Rabbit IgG isotype control (2.5 µg/ml, blue line) or Anti-CD3G Antibody [ARC2105] (A306075), (2.5 µg/ml orange line), followed by goat anti-Rabbit polyclonal antibody FITC (1:200 dilution) staining. Non-fluorescently stained Raji cells was used as blank control (red line).