Anti-CD35 Antibody [E11] - BSA and Azide free (A280300)

Anti-CD35 Antibody [E11] - BSA and Azide free

Mouse monoclonal [E11] antibody to CD35.

This antibody recognises complement receptor type 1, also known as CD35 or the C3b/C4b receptor. CD35 a single chain, type I single pass transmembrane glycoprotein which exists in four allotypic forms (A,B,C,D) of ~190 kDa, ~220 kDa, ~160 kDa and ~250 kDa respectively. CD35 is expressed by granulocytes, monocytes, B cells and some T cells. Functionally, CD35 acts as a receptor for the C3b component of complement. Mouse anti Human CD35 antibody, clone E11 is suitable for use in Western Blotting.

IHC-Fr, IHC-P, Flow Cytometry

Flow Cytometry: 1:50 - 1:200, IHC-Fr: 1 µg/ml - 10 µg/ml, IHC-P: 1 µg/ml - 10 µg/ml, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood.

Human, Cynomolgus monkey, Rhesus Monkey, Baboon

Human acute monocytic leukaemia cells.

Mouse

Monoclonal

E11

IgG1

Protein A affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Human CD35 antibody, clone E11 recognizes complement receptor type 1, also known as CD35 or the C3b/C4b receptor. CD35 a single chain, type I single pass transmembrane glycoprotein which exists in four allotypic forms (A,B,C,D) of ~190 kDa, ~220 kDa, ~160 kDa and ~250 kDa respectively (Khera et al. 2009). CD35 is expressed by granulocytes, monocytes, B cells and some T cells. Functionally, CD35 acts as a receptor for the C3b component of complement.Mouse anti Human CD35 antibody, clone E11 is suitable for use in Western Blotting (Birmingham et al. 1996).

C3b/C4b receptor, C3BR, Complement receptor type 1, CR1