Anti-CD34 Antibody [QBEND/10] (PE) (A281615)

$205

Mouse monoclonal [QBEND/10] antibody to CD34 (PE) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-CD34 Antibody [QBEND/10] (PE)
Description
Mouse monoclonal [QBEND/10] antibody to CD34 (PE).
Specificity
This antibody recognises the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells and small vessel endothelium Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays. Mouse anti Human CD34 antibody, clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Mouse anti Human CD34 antibody, clone QBEND/10 binds to a different eoptope to Mouse anti Human CD34, clone 581 which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte Typing VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it's binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typing VI). Mouse anti Human CD34 antibody, clone QBEND/10 has been successfully exploited for the detection of CD34 in brain capillaries of Alzheimer's patients and in acute lymphoblastic leukemia cells by western blotting.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat - 1:10 Pack Size: 100 TestsNeat Pack Size: 25 Tests/0.25ml, Use 10µl of the suggested working dilution to label 1 x 106 cells in 100µl.
Reactivity
Human, Cynomolgus monkey, Rhesus Monkey
Cross Reactivity
This antibody does not cross react with Bovine, Sheep, Rat, or Dog
Immunogen
Human endothelial cell membrane vesicles.
Host
Mouse
Clonality
Monoclonal
Clone ID
QBEND/10
Isotype
IgG1
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Pack Size of 100 Tests: Reconstitute with 1 ml of distilled water. Pack Size of 25 Tests/0.25ml: Reconstitute with 0.25 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Lyophilized: Store at 4°C. Reconstituted: Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells (Kaufman et al. 2001) and small vessel endothelium (Ramani et al. 1990)Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanza et al. 1999). Mouse anti Human CD34 antibody, clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Mouse anti Human CD34 antibody, clone QBEND/10 binds to a different eoptope to Mouse anti Human CD34, clone 581 which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte Typing VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it's binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typing VI).Mouse anti Human CD34 antibody, clone QBEND/10 has been successfully exploited for the detection of CD34 in brain capillaries of Alzheimer's patients (Kalaria et al. 1992) and in acute lymphoblastic leukemia cells (Sutherland et al. 1992) by western blotting.
Synonyms
Hematopoietic progenitor cell antigen CD34
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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