Anti-CD34 Antibody [MEC14.7] - BSA and Azide free (A281083)

Anti-CD34 Antibody [MEC14.7] - BSA and Azide free

Rat monoclonal [MEC14.7] antibody to CD34.

This antibody recognises the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.

IHC-P, IP, Flow Cytometry

Flow Cytometry: 1:10 for 0.25 mg, 25 µg, 1:25 for 0.1 mg - 1:20 for 0.25 mg, 25 µg, 1:50 for 0.1 mg, IHC-P: 1:20 - 1:200, IP: 2 µg/ml - 10 µg/ml, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse

T-end.1, a pMT transformed endothelial cell line.

Rat

Monoclonal

MEC14.7

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.

Hematopoietic progenitor cell antigen CD34