Anti-CD34 Antibody [MEC14.7] (A281082)

Anti-CD34 Antibody [MEC14.7]

Rat monoclonal [MEC14.7] antibody to CD34.

This antibody recognises the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. recognises a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.

IHC-P, IP, Flow Cytometry

Flow Cytometry: 1:10 for 0.25 mg, 25 µg, 1:25 for 0.1 mg - 1:20 for 0.25 mg, 25 µg, 1:50 for 0.1 mg, IHC-P: 1:20 - 1:200, IP: 2 µg/ml - 10 µg/ml, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse

T-end.1, a pMT transformed endothelial cell line.

Rat

Monoclonal

MEC14.7

IgG2a

Protein G affinity chromatography of tissue culture supernatant.

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes the murine CD34 cell surface antigen, expressed by endothelial cells and by hematopoietic stem cells. Rat anti Mouse CD34 antibody, clone MEC14.7 recognizes a neuraminidase sensitive epitope. As in the human system, CD34 antibodies in the mouse demonstrate slightly different staining patterns depending on their fine specificity. Rat anti Mouse CD34 antibody, clone MEC14.7 appears to recognize a subset of the stem cell population recognized by clone RAM34, and it is thought that this is due to differences in the epitope recognized by the two antibodies.

Hematopoietic progenitor cell antigen CD34