Anti-CD31 Antibody [TLD-3A12] - BSA and Azide free (A281834)

Anti-CD31 Antibody [TLD-3A12] - BSA and Azide free

Mouse monoclonal [TLD-3A12] antibody to CD31.

This antibody recognises rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque

Flow Cytometry, WB, ELISA, IHC-Fr, IF

Flow Cytometry: 1:10 - 1:100, IHC-Fr: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 10⁶ cells in 100µl

Rat, Rhesus Monkey, Pig

Activated, Lewis rat derived microglial cells.

Mouse

Monoclonal

TLD-3A12

IgG1

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001)

EndoCAM, GPIIA', PECA1, PECAM-1, PECAM1, Platelet endothelial cell adhesion molecule