Anti-CD31 Antibody [TLD-3A12] (A281835)

$340

Mouse monoclonal [TLD-3A12] antibody to CD31 for WB, Flow Cytometry, IHC-Fr, ELISA and IF.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 4-6 business days.
Name
Anti-CD31 Antibody [TLD-3A12]
Description
Mouse monoclonal [TLD-3A12] antibody to CD31.
Specificity
This antibody recognises rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque
Applications
WB, Flow Cytometry, IHC-Fr, ELISA, IF
Dilutions
Flow Cytometry: 1:10 - 1:100, IHC-Fr: 1:10 - 1:100, Use 10µl of the suggested working dilution to label 106 cells in 100µl
Reactivity
Rat, Rhesus Monkey, Pig
Immunogen
Activated, Lewis rat derived microglial cells.
Host
Mouse
Clonality
Monoclonal
Clone ID
TLD-3A12
Isotype
IgG1
Conjugate

Unconjugated

Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Rat CD31 antibody, clone TLD-3A12 recognizes rat PECAM-1 (CD31), a 661 amino acid type 1 transmembrane protein expressed primarily on endothelial cells, platelets and leucocytes.Clone TLD-3A12 has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen (Stevenson, K.S. et al.2009). Mouse anti Rat CD31 antibody, clone TLD-3A12 is suitable for use in IHC on formalin-fixed paraffin-embedded sections pre-treated with 0.2M boric acid, pH7.0. (Wilson et al. 2007). Mouse anti Rat CD31, clone TLD-3A12 has been shown to be cross-reactive with endothelial cells derived from rhesus macaque (Maclean et al. 2001)
Synonyms
EndoCAM, GPIIA', PECA1, PECAM-1, PECAM1, Platelet endothelial cell adhesion molecule
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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