Anti-CD31 Antibody [LCI-4] (Biotin) (A280860)

Anti-CD31 Antibody [LCI-4] (Biotin)

Mouse monoclonal [LCI-4] antibody to CD31 (Biotin).

This antibody recognises porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types. The extracellular region contains six Ig-like domains. Mouse anti Pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants. Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions.

Flow Cytometry: Neat - 1:200, Use 10µl of the suggested working dilution to label 10⁶ cells or 100µl whole blood

Porcine, Human

This antibody does not cross react with Mouse

Porcine CD31/human IgGFc fusion protein.

Mouse

Monoclonal

LCI-4

Protein A affinity chromatography of tissue culture supernatant.

500 µg/ml

Liquid

Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light.

Mouse anti Pig CD31, clone LCI-4 recognizes porcine CD31, also known as Platelet endothelial cell adhesion molecule (PECAM-1). CD31 is constitutively expressed by platelets, monocytes and some lymphocytes, it is expressed by endothelial cells at a level, an order of magnitude greater that of other cell types (Fawcwett et al.1995). The extracellular region contains six Ig-like domains. Mouse anti Pig CD31, clone LCI-4 is cross reactive with human CD31 and binds to the 5th extracellular Ig domain, proximal to the transmembrane region as demonstrated by human CD31 domain deletion mutants (Nasu et al.1999). Mouse anti Pig CD31, clone LCI-4 immunoprecipitates a protein of ~130 kDa from lysates of porcine aortic endothelial cells and is strongly expressed at cell junctions (Nasu et al. 1999).

EndoCAM, GPIIA', PECA1, PECAM-1, PECAM1, Platelet endothelial cell adhesion molecule

This product is for research use only. It is not intended for diagnostic or therapeutic use.