Anti-CD31 Antibody [ER-MP12] (A280366)

Anti-CD31 Antibody [ER-MP12]

Rat monoclonal [ER-MP12] antibody to CD31.

This antibody recognises mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow.

IP, Flow Cytometry, IHC-Fr, IF

Flow Cytometry: 1:50 for 25 µg, 0.25 mg, 1:10 for 0.1 mg - 1:200 for 25 µg, 0.25 mg, 1:100 for 0.1 mg, IHC-Fr 1: 1:10 - 1:100, Use10µl of the suggested working dilution to label 10⁶ cells in 100µl

Mouse

BALB/c macrophage precursor cell hybrids.

Rat

Monoclonal

ER-MP12

IgG2a

1 mg/ml

Liquid

Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.

Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.

Rat anti Mouse CD31 antibody, clone ER-MP12 recognizes mouse CD31, a 140 kDa cell surface glycoprotein expressed at high levels on endothelial cells, platelets and most leukocyte subpopulations.CD31 is also expressed on a major population of macrophage / dendritic cell precursors in the bone marrow. Rat anti Mouse CD31 antibody, clone ER-MP12 can be used in conjunction with clone ER-MP20 (MCA2389GA) in two colour flow cytometric analysis, to identify different stages of myeloid progenitor cells in mouse bone marrow (de Bruijn et al. 1998).

EndoCAM, GPIIA', PECA1, PECAM-1, PECAM1, Platelet endothelial cell adhesion molecule