This antibody recognises ovine CD31, also known as PECAM-1.Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells. Mouse anti Sheep CD31 antibody, clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies.
Applications
IP, Flow Cytometry
Dilutions
Flow Cytometry: 1:10 - 1:25, Use 10µl of the suggested working dilution to label 1 x 106 cells in 100µl
Reactivity
Sheep, Goat, Bovine
Immunogen
Sheep leucocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
CO.3E1D4
Isotype
IgG2a
Conjugate
Unconjugated
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline with 0.09% Sodium Azide.
Storage
Shipped at ambient temperature. Upon delivery aliquot and store at -20°C. When thawed, aliquot the sample as needed. Short term (up to 4 weeks): store at 4°C. Long term: store at -20°C. Avoid freeze / thaw cycles. Storage in frost free freezers is not recommended.
General Notes
Mouse anti Sheep CD31 antibody, clone CO.3E1D4 recognizes ovine CD31, also known as PECAM-1.Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells.Mouse anti Sheep CD31 antibody, clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies (Pintado et al. 1995).