Anti-CD31 Antibody [2H8] - BSA and Azide free (A279243)

Anti-CD31 Antibody [2H8] - BSA and Azide free

Hamster monoclonal [2H8] antibody to CD31.

This antibody recognises mouse CD31, also known as Platelet endothelial cell adhesion molecule or PECAM-1. CD31 is a ~130kDa single pass type-1 membrane glycoprotein bearing six C2 Ig-like domains, expressed on all continuous endothelium including arteries, veins and non-sinusoidal capillaries. CD31 is also expressed on all haemopoietic lineages with the exception of the erythroid line. Hamster anti mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation. CD31 has also been shown to be critically involved in the sensing of changes in shear stress associated with atherosclerotic lesions and in the associated atherogenesis.

Flow Cytometry, IF, Functional Assays

Flow Cytometry: 0.1 µg/ml, Use 5µl of the suggested working dilution to label 100µl of whole blood

Mouse

D10.G4.1 cells.

Hamster

Monoclonal

2H8

IgG

Protein G affinity chromatography of ascites.

500 µg/ml

Sterile filtered.

Liquid

Supplied in Phosphate Buffered Saline.

Store undiluted at -20°C only. Storage in frost free freezers is not recommended. Avoid freeze-thaw cycles. Should this product contain a precipitate we recommend microcentrifugation before use.

Hamster anti Mouse CD31 monoclonal antibody, clone 2H8 recognizes murine CD31, also known as Platelet endothelial cell adhesion molecule or PECAM-1. CD31 is a ~130kDa single pass type-1 membrane glycoprotein bearing six C2 Ig-like domains, expressed on all continuous endothelium including arteries, veins and non-sinusoidal capillaries. CD31 is also expressed on all haemopoietic lineages with the exception of the erythroid line (Bogen et al. 1992) . Hamster anti mouse CD31, clone 2H8 effectively inhibits transmigration of activated polymorphonuclear cells and monocytes across the endothelium. In a mouse model for acute peritonitis clone 2H8 blocks acute inflammation (Bogen et al. 1994). CD31 has also been shown to be critically involved in the sensing of changes in shear stress associated with atherosclerotic lesions and in the associated atherogenesis (Stevens et al. 2008).

EndoCAM, GPIIA', PECA1, PECAM-1, PECAM1, Platelet endothelial cell adhesion molecule