Anti-CD3 epsilon Antibody [C3e/1308] (A250505)

Anti-CD3 epsilon Antibody [C3e/1308]

Mouse monoclonal [C3e/1308] antibody to CD3 epsilon.

This antibody recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

WB, Flow Cytometry

WB: 1-2 µg/ml, Flow Cytometry: 1-2 µg/million cells

Human

Recombinant fragment, around amino acids 23-119, of human CD3e protein. The exact sequence is proprietary.

Mouse

Monoclonal

C3e/1308

IgG2b

Protein A/G chromatography.

200 µg/ml

20 kDa

Liquid

Supplied in 10mM Phosphate Buffered Saline with 0.05% BSA and 0.05% Sodium Azide.

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

This monoclonal antibody is also available in a different formulation without BSA and Sodium Azide - Anti-CD3 epsilon Antibody [C3e/1308] - BSA and Azide free (A253685).

CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E