Anti-CD3 epsilon Antibody [APA1/1] (PE) (A85739)

Anti-CD3 epsilon Antibody [APA1/1] (PE)

Mouse monoclonal [APA1/1] antibody to CD3 epsilon (PE).

This antibody recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.

Flow Cytometry: 1-4 µg/ml; Intracellular staining, IHC: Fixed and permeabilised cells. The antibody can distinguish TCR-stimulated from non-stimulated cells.

Mouse, Human

Purified human CD3 proteins isolated from thymus.

Mouse

Monoclonal

IgG1

This antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography; removing unconjugated antibody and free fluorochrome.

100 µg/ml

Liquid

Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.

Shipped at 4°C. Store in the dark at 2-8°C. Do not freeze!

CD3e, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E

This product is for research use only. It is not intended for diagnostic or therapeutic use.