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Anti-CD3 Antibody [MEM-57] (PE) (A281165)

$535

Mouse monoclonal [MEM-57] antibody to CD3 (PE) for Flow Cytometry.

Alternative Formats and ConjugatesAlternative Formats

Shipping Information

$40
Dispatched from St. Louis, MO.
Lead Time: 5-7 business days.
Name
Anti-CD3 Antibody [MEM-57] (PE)
Description
Mouse monoclonal [MEM-57] antibody to CD3 (PE).
Specificity
This antibody recognises a conformational CD3 epitope minimally requiring the presence of the CD3e subunit extracellular domain as it will recognize murine 3D054.8 hybridoma cells transfected with cDNA to express the human CDe subunit lacking its cytoplasmic domain. CD3 serves as a pan T cell marker, it is expressed on the surface of over 95% of circulating human peripheral T cells. CD3 is also present on ~60-80% of thymocytes and on Purkinji cells, neurons present in the cerebellar cortex. CD3 is not expressed on B cells or NK cells. The TCR complex consists of TCR heterodimers, either a/ß or ?/d expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, ?, d, e and ? responsible for effective signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhns et al. 2006, Dave et al. 1997a, Dave et al. 1997b). Mouse anti Human CD3 clone MEM-57 demonstrates a mitogenic effect on peripheral blood T cells and on the lymphoblastoid Jurkat cell line (Batista et al. 2004 Brdicková et al. 2003). Clone MEM-57 immunoprecipitates proteins of ~22 kDa and more weakly ~28 kDa. from peripheral blood T cell lysates.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells in 100µl or 100µl whole blood
Reactivity
Human, Chimpanzee
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-57
Isotype
IgG2a
Conjugate

PE

Excitation: 565nm, Emission: 578nm

Purification
Protein G affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Mouse anti Human CD3 (MEM-57) is a monoclonal antibody recognizing an extracellular epitope of the human CD3 complex containing either d-e or ?-e subunit complexes (Dave et al. 1997). Mouse anti Human CD3 (MEM-57) recognizes a conformational CD3 epitope minimally requiring the presence of the CD3e subunit extracellular domain (Transy et al. 1989) as it will recognize murine 3D054.8 hybridoma cells transfected with cDNA to express the human CDe subunit lacking its cytoplasmic domain. CD3 serves as a pan T cell marker, it is expressed on the surface of over 95% of circulating human peripheral T cells. CD3 is also present on ~60-80% of thymocytes and on Purkinji cells, neurons present in the cerebellar cortex. CD3 is not expressed on B cells or NK cells. The TCR complex consists of TCR heterodimers, either a/ß or ?/d expressed in a mutually exclusive manner, analagous that seen in mice on different T cell lineages. In order for the TCR heterodimer to be expressed on the cell surface it must associate with a minimal CD3 complex. The CD3 complex itself is made up of four subunits, ?, d, e and ? responsible for effective signalling upon TCR activation, via a single or multiple, depending on the subunit, immunoreceptor tyrosine-based activation motif (ITAM) present in the CD3 subunit cytoplasmic domain (Kuhns et al. 2006, Dave et al. 1997a, Dave et al. 1997b).Mouse anti Human CD3 clone MEM-57 demonstrates a mitogenic effect on peripheral blood T cells and on the lymphoblastoid Jurkat cell line (Batista et al. 2004 Brdicková et al. 2003). Clone MEM-57 immunoprecipitates proteins of ~22 kDa and more weakly ~28 kDa. from peripheral blood T cell lysates.
Synonyms
CD3d, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain, T3D
Disclaimer
This product is for research use only. It is not intended for diagnostic or therapeutic use.
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