This antibody reacts with an extracellular epitope on gamma-epsilon and delta-epsilon dimers of human CD3 complex, a part of a bigger multi-subunit T cell receptor complex (CD3/TCR) expressed on peripheral blood T lymphocytes and mature thymocytes.
Applications
Flow Cytometry, IP, CyTOF
Dilutions
IP: The antibody MEM-57 immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22 kDa and a weak 28-kDa zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7), Flow Cytometry: 2 - 5 µg/ml; Positive Control: peripheral blood lymphocytes, JURKAT human leukemia T cell line.
Reactivity
Human
Immunogen
Human thymocytes and T lymphocytes.
Host
Mouse
Clonality
Monoclonal
Clone ID
MEM-57
Isotype
IgG2a
Light Chains
kappa
Conjugate
Unconjugated
Purification
Protein A chromatography.
Concentration
1 mg/ml
Product Form
Liquid
Formulation
Supplied in Phosphate Buffered Saline, pH 7.4, with 15 mM Sodium Azide.
Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze/thaw cycles.