This antibody recognises the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.
Applications
Flow Cytometry
Dilutions
Flow Cytometry: Neat, Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).
Reactivity
Mouse
Immunogen
CBAT6 thymocytes.
Host
Rat
Clonality
Monoclonal
Clone ID
KT3
Isotype
IgG2a
Conjugate
PE
Excitation: 565nm, Emission: 578nm
Purification
Protein A affinity chromatography of tissue culture supernatant.
Concentration
Reconstitution dependent.
Product Form
Lyophilized
Reconstitution
Reconstitute with 1 ml of distilled water.
Formulation
Supplied in Phosphate Buffered Saline with 1% BSA, 5% Sucrose, and 0.09% Sodium Azide.
Storage
Store undiluted at 4°C. Do not freeze! This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
General Notes
Rat anti Mouse CD3 antibody, clone KT3 recognizes the mouse CD3 antigen, expressed by mature T cells. Rat anti Mouse CD3 antibody, clone KT3 may be used to trigger proliferation and cytotoxicity of CD3 positive cells (Tomonari 1988).NB. For optimal staining incubations should be performed at room temperature.